10.6084/m9.figshare.1000733.v2 C. T. Sulaiman C. T. Sulaiman Indira Balachandran Indira Balachandran Chemical Profiling of an Indian Herbal Formula Using Liquid Chromatography Coupled with Electro Spray Ionization Mass Spectrometry Taylor & Francis Group 2014 chemical Profiling identification gallic acid plant parts Liquid chromatography phenolic acids protocatechuic acid ms zingiber officinale electro spray ionization mass spectrometry Tinospora cordifolia Electro Spray Ionization Mass Spectrometry Amruthotharam kashayam chebulic acid mass spectrum Indian Herbal Formula chemical fingerprint quinic acid Ayurvedic formulation Terminalia chebula 2014-10-30 17:52:27 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Chemical_Profiling_of_an_Indian_Herbal_Formula_Using_Liquid_Chromatography_Coupled_with_Electro_Spray_Ionization_Mass_Spectrometry/1000733 <div><p> <i>Amruthotharam kashayam</i> is an important <i>Ayurvedic</i> formulation prepared using specified plant parts of <i>Tinospora cordifolia, Terminalia chebula</i>, and <i>Zingiber officinale.</i> The current study developed a rapid liquid chromatographic method coupled with electro spray ionization mass spectrometry for the identification of major phytoconstituents present in the formulation. Reverse phase high-pressure liquid chromatogram was developed as chemical fingerprint. The mass spectrum along with the MS/MS fragmentation on collision-induced dissociation led to the structural identification of separated compounds. Phenolic acids such as quinic acid, protocatechuic acid, gallic acid, and chebulic acid were identified in the formulation along with some flavonoids.</p> </div>