J. Lyu, S. D. Tian, Y. H. Wang, S. L. Han, R. X. Mei, X. T. Kang, X. A novel 2-bp indel within Krüppel-like factor 15 gene (<i>KLF15</i>) and its associations with chicken growth and carcass traits <div><p></p><p>1. The Krüppel-like factor (KLF) family of zinc-finger transcription factors plays a critical role in cell differentiation, phenotypic modulation and physiologic function. <i>KLF15</i> has been proposed to regulate adipogenesis and gluconeogenesis. The objective of this study was to establish the association between <i>KLF15</i> gene polymorphism and chicken growth and carcass traits.</p><p>2. An F<sub>2</sub>resource population of Gushi chickens crossed with Anka broilers was used to investigate the genetic effects of the chicken <i>KLF15</i> gene. A 2-bp indel mutation (G13781_13782del/insAG) within intron 2 was detected, and a polymerase chain reaction–restriction fragment length polymorphism method was developed to genotype the F<sub>2</sub> individuals.</p><p>3. Association analysis showed that the Single Nucleotide Polymorphisms (SNP) was significantly associated with chicken growth and carcass traits. The chickens with the insAG/insAG genotype generally had a significantly higher body weight and size than other genotypes. Gene expression for each genotype showed that birds carrying insAG/insAG had a higher expression level than the other genotypes.</p><p>4. The results suggested that this polymorphic site may serve as a useful target for marker assisted selection of chicken growth and carcass traits.</p></div> Single Nucleotide Polymorphisms;factor;SNP;Genotype;carcass traits;F 2resource population;carcass traits 1.;F 2 individuals .3. Association analysis;KLF 15 gene polymorphism;carcass traits .2.;chicken growth;chicken KLF 15 gene 2014-11-17
    https://tandf.figshare.com/articles/dataset/A_novel_2_bp_indel_within_Kr_ppel_like_factor_15_gene_i_KLF15_i_and_its_associations_with_chicken_growth_and_carcass_traits/1116232
10.6084/m9.figshare.1116232.v2