Mir-Cros, Alba Moreno-Mingorance, Albert Martín-Gómez, M. Teresa Codina, Gema Cornejo-Sánchez, Thais Rajadell, Mireia Van Esso, Diego Rodrigo, Carlos Campins, Magda Jané, Mireia Pumarola, Tomàs Fàbrega, Anna González-López, Juan José Population dynamics and antigenic drift of <i>Bordetella pertussis</i> following whole cell vaccine replacement, Barcelona, Spain, 1986–2015 <p>Among the factors associated with the resurgence of whooping cough, special emphasis has been given to pathogen adaptation after the introduction of the acellular vaccine (ACV). To assess the impact of the vaccine transition strategy from whole-cell vaccine (WCV) to ACV on population dynamics of <i>Bordetella pertussis</i> in Barcelona (Spain), we studied 339 isolates collected from 1986 to 2015 by PFGE and multi-locus variable-number tandem repeat analysis (MLVA). Additionally, allelic variants for the pertussis toxin and its promoter, pertactin, type 3 fimbriae and fimbrial serotyping were assessed to determine its antigenic drift. A shift was observed in the <i>B. pertussis</i> population as well as in its antigenic profile concurrently with the introduction of ACV in Barcelona. Four out of the five most prevalent PFGE profiles were replaced by new profiles following the ACV introduction. MLVA type 27 was the dominant genotype, and its frequency increased from 25% to 79.3% after WCV replacement. Antigen typing demonstrated the emergence of <i>prn2</i>, <i>ptxP3</i>, <i>fim3-2</i> and a shift from the fimbriae 3 to the fimbriae 2 serotypes after the ACV introduction. Our findings support the presence of population and antigenic dynamic changes in <i>B. pertussis</i> likely driven by the introduction of ACV.</p> Whooping cough;PFGE;MLVA;antigenic variants;pertussis vaccine 2019-11-26
    https://tandf.figshare.com/articles/dataset/Population_dynamics_and_antigenic_drift_of_i_Bordetella_pertussis_i_following_whole_cell_vaccine_replacement_Barcelona_Spain_1986_2015/11205380
10.6084/m9.figshare.11205380.v1