A new triterpene and protective effect of <i>Periploca somaliensis</i> Browicz fruits against CCl<sub>4</sub>-induced injury on human hepatoma cell line (Huh7)
Azza R. Abdel-Monem
Zeinab A. Kandil
Ashraf B. Abdel-Naim
Essam Abdel-Sattar
10.6084/m9.figshare.1157756.v2
https://tandf.figshare.com/articles/journal_contribution/A_new_triterpene_and_protective_effect_of_i_Periploca_somaliensis_i_Browicz_fruits_against_CCl_sub_4_sub_induced_injury_on_human_hepatoma_cell_line_Huh7_/1157756
<div><p>The potential hepatoprotective effect of the methanolic extract of <i>Periploca somaliensis</i> Browicz fruits, its different fractions (<i>n</i>-hexane, chloroform and <i>n</i>-butanol) and the major isolated compound ursolic acid was evaluated using the human hepatoma cell line (Huh7) based on the changes in the activity of aspartate aminotransferase, alanine transaminase, glutathione and superoxide dismutase. Each sample was tested at three different concentrations (1000, 100 and 10 μg/mL). All tested samples exhibited a potent concentration-independent cytoprotective effect relative to silymarin as a reference standard. Chromatographic fractionation of the chloroform-soluble fraction of the methanol extract of <i>P. somaliensis</i> Browicz fruits afforded two known triterpenes, namely ursolic acid, and 11α,12α-epoxy-3β-hydroxy-olean-13β,28-olide, and a newly discovered one, namely 3β-hydroxy-urs-11-en-13β,28-olide. The structures of the isolated compounds were elucidated by the analysis of 1D and 2D NMR spectral data.</p></div>
2015-01-08 11:19:20
compound ursolic acid
aspartate aminotransferase
superoxide dismutase
sample
triterpene
ursolic acid
Huh
somaliensis Browicz fruits
alanine transaminase
1 D
Periploca somaliensis Browicz fruits
hepatoprotective effect
hepatoma cell line
Chromatographic fractionation
2 D NMR