Anti-inflammatory isocoumarins from the bark of <i>Fraxinus chinensis</i> subsp. <i>rhynchophylla</i> Beom Zoo Lee Chan-Sik Kim Soon-Kyu Jeong Sulhae Lee Ik Soo Lee KwangWon Hong 10.6084/m9.figshare.11672280.v1 https://tandf.figshare.com/articles/journal_contribution/Anti-inflammatory_isocoumarins_from_the_bark_of_i_Fraxinus_chinensis_i_subsp_i_rhynchophylla_i_/11672280 <p>A new isocoumarin (<b>1</b>) named fraxicoumarin was isolated from the bark of <i>Fraxinus chinensis</i> subsp. <i>rhynchophylla</i> along with three known compounds (<b>2</b>–<b>4</b>). The structure of the new compound was established by extensive spectroscopic studies and chemical evidence. The anti-inflammatory effects of the isolated compounds (<b>1</b>–<b>4</b>) on lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cells were evaluated <i>in vitro</i>. Of the compounds tested, compounds <b>1</b> and <b>3</b> inhibited LPS-induced nitric oxide (NO) production in RAW 264.7 cells. Consistent with these findings, they also suppressed LPS-induced expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the protein level in RAW 264.7 cells.</p> <p></p> 2020-01-21 11:53:37 Fraxinus chinensis subsp. rhynchophylla isocoumarins anti-inflammation nitric oxide inducible nitric oxide synthase cyclooxygenase-2