%0 Journal Article %A Yamasaki, Masahiro %A Hasegawa, Shinya %A Takahashi, Hiroshi %A Kobayashi, Yuka %A Sakai, Chihiro %A Ashizawa, Yoko %A Asai, Yuuki %A Kanzaki, Mamiko %A Fukui, Tetsuya %D 2015 %T Placental extracts induce the expression of antioxidant enzyme genes and suppress melanogenesis in B16 melanoma cells %U https://tandf.figshare.com/articles/journal_contribution/Placental_extracts_induce_the_expression_of_antioxidant_enzyme_genes_and_suppress_melanogenesis_in_B16_melanoma_cells/1254795 %R 10.6084/m9.figshare.1254795.v2 %2 https://tandf.figshare.com/ndownloader/files/1810292 %K PE treatment %K B 16 cells %K B 16 melanoma cells %K sod %K antioxidant enzyme genes %K H 2O cell death %K cytosolic superoxide dismutase %K expression %X

One of the activities of placental extracts (PEs) is skin-whitening effect, but the physiological and genetic mechanism for this effect has not yet been clarified. Here, we focus on PE as a regulator of antioxidant enzyme genes. Porcine PE was prepared, and its activity was investigated in B16 melanoma cells. PE treatment decreased the melanin content of UV-irradiated B16 cells in a dose-dependent manner. PE directly reduced the enzyme activity of tyrosinase in a cell-free assay. In addition, PE treatment increased the gene expression of cytosolic superoxide dismutase (SOD-1), extracellular SOD (SOD-3) and catalase but did not affect the expression of tyrosinase. Moreover, PE protected the B16 cells from H2O2-induced cell death. Taken together, our data suggest that PEs could play a role not only as a suppressor of melanin synthesis but also as a regulator of antioxidant genes and might protect the skin against oxidative stress.

%I Taylor & Francis