Yamasaki, Masahiro Hasegawa, Shinya Takahashi, Hiroshi Kobayashi, Yuka Sakai, Chihiro Ashizawa, Yoko Asai, Yuuki Kanzaki, Mamiko Fukui, Tetsuya Placental extracts induce the expression of antioxidant enzyme genes and suppress melanogenesis in B16 melanoma cells <div><p>One of the activities of placental extracts (PEs) is skin-whitening effect, but the physiological and genetic mechanism for this effect has not yet been clarified. Here, we focus on PE as a regulator of antioxidant enzyme genes. Porcine PE was prepared, and its activity was investigated in B16 melanoma cells. PE treatment decreased the melanin content of UV-irradiated B16 cells in a dose-dependent manner. PE directly reduced the enzyme activity of tyrosinase in a cell-free assay. In addition, PE treatment increased the gene expression of cytosolic superoxide dismutase (SOD-1), extracellular SOD (SOD-3) and catalase but did not affect the expression of tyrosinase. Moreover, PE protected the B16 cells from H<sub>2</sub>O<sub>2</sub>-induced cell death. Taken together, our data suggest that PEs could play a role not only as a suppressor of melanin synthesis but also as a regulator of antioxidant genes and might protect the skin against oxidative stress.</p></div> PE treatment;B 16 cells;B 16 melanoma cells;sod;antioxidant enzyme genes;H 2O cell death;cytosolic superoxide dismutase;expression 2015-10-08
    https://tandf.figshare.com/articles/journal_contribution/Placental_extracts_induce_the_expression_of_antioxidant_enzyme_genes_and_suppress_melanogenesis_in_B16_melanoma_cells/1254795
10.6084/m9.figshare.1254795.v2