10.6084/m9.figshare.1286778.v2
S. Perumal
S.
Perumal
R. Mahmud
R.
Mahmud
S. Ramanathan
S.
Ramanathan
Anti-infective potential of caffeic acid and epicatechin 3-gallate isolated from methanol extract of <i>Euphorbia hirta</i> (L.) against <i>Pseudomonas aeruginosa</i>
Taylor & Francis Group
2015
caffeic acid
Pseudomonas aeruginosa Euphorbia hirta
ecg
epicatechin
gallate
broth microdilution method
methanol
ca
compound
2015-08-04 14:27:20
Journal contribution
https://tandf.figshare.com/articles/journal_contribution/Anti_infective_potential_of_caffeic_acid_and_epicatechin_3_gallate_isolated_from_methanol_extract_of_i_Euphorbia_hirta_i_L_against_i_Pseudomonas_aeruginosa_i_/1286778
<div><p><i>Euphorbia hirta</i> (L.) plant is traditionally used in Malaysia for the treatment of gastrointestinal, bronchial and respiratory ailments caused by nosocomial infectious agents. Bioactivity-guided fractionation of the methanol extract of the aerial parts of <i>E. hirta</i> and analysis using high-performance liquid chromatography have led to the isolation of two antibacterial compounds. These compounds were identified as caffeic acid (CA) and (–)-epicatechin 3-gallate (ECG) based on spectroscopic analyses and comparison with previously published data. Using broth microdilution method, both ECG and CA had demonstrated significant minimum inhibitory concentration of 15.6 and 31.3 μg/mL respectively, against <i>Pseudomonas aeruginosa</i>. Time-kill assessment of ECG and CA displayed bactericidal effect on <i>P. aeruginosa</i> cells.</p></div>