%0 Journal Article %A B. Pietsch, Renée %A F. David, Ray %A Marr, Linsey C. %A Vinatzer, Boris %A G. Schmale III, David %D 2015 %T Aerosolization of Two Strains (Ice+ and Ice–) of Pseudomonas syringae in a Collison Nebulizer at Different Temperatures %U https://tandf.figshare.com/articles/journal_contribution/Aerosolization_of_Two_Strains_Ice_and_Ice_of_i_Pseudomonas_syringae_i_in_a_Collison_Nebulizer_at_Different_Temperatures/1301650 %R 10.6084/m9.figshare.1301650.v5 %2 https://tandf.figshare.com/ndownloader/files/1880791 %K future work %K strain %K Collison Nebulizer %K laboratory conditions %K aerosol production %K aerosolization %K ice nucleation %K Pseudomonas syringae %K bacterium Pseudomonas syringae %K Different Temperatures %K water temperatures %K systems.Copyright 2015 American Association %K ice nucleation phenotype %K nonice nucleation %X

The aerosolization of microorganisms from aquatic environments is understudied. This article describes a study in which an ice nucleation active (ice+) strain and a nonice nucleation active (ice−) strain of the bacterium Pseudomonas syringae were aerosolized from aqueous suspensions under artificial laboratory conditions using a collison nebulizer. The aerosolization of P. syringae was not influenced by water temperatures between 5° and 30°C. In general, the culturability (viability) of P. syringae in aerosols increased with temperature between 5 and 30°C. The ice+ strain was aerosolized in greater numbers than the ice– strain at all temperatures studied, suggesting a possible connection between the ice nucleation phenotype and aerosol production. Together, our results suggest that P. syringae has the potential to be aerosolized from natural aquatic environments, such as streams, rivers, ponds, and lakes; known reservoirs of P. syringae. Future work is needed to elucidate the mechanisms of aerosolization of P. syringae from natural aquatic systems.

Copyright 2015 American Association for Aerosol Research

%I Taylor & Francis