10.6084/m9.figshare.1418032.v1
Yi Li
Yi
Li
Yongxin Zhang
Yongxin
Zhang
Xiaowei Zheng
Xiaowei
Zheng
Liping Sun
Liping
Sun
Yitao Qi
Yitao
Qi
Joan Ritho
Joan
Ritho
Jinhui Zhou
Jinhui
Zhou
Characterization of flavonoid glycosides from rapeseed bee pollen using a combination of chromatography, spectrometry and nuclear magnetic resonance with a step-wise separation strategy
Taylor & Francis Group
2015
strategy
Flavonoid Glycosides
chromatography
separation
resonance
kaempferol
lc
nmr
spectrometry
rape bee pollen samples
rapeseed bee pollen
2015-05-18 00:00:00
Journal contribution
https://tandf.figshare.com/articles/journal_contribution/Characterization_of_flavonoid_glycosides_from_rapeseed_bee_pollen_using_a_combination_of_chromatography_spectrometry_and_nuclear_magnetic_resonance_with_a_step_wise_separation_strategy/1418032
<div><p>To identify the structures of flavonoid glycosides in bee pollen collected from rapeseed plants (<i>Brassica napus</i> L.), we utilised an approach that combined liquid chromatography–diode array detector–electrospray ionization–mass spectrometry (LC–DAD–ESI–MS) and nuclear magnetic resonance (NMR) technology with a step-wise separation strategy. We identified four constituents of high purity in rape bee pollen samples: (1) quercetin-3-<i>O</i>-β-D-glucosyl-(2→l)-β-glucoside, (2) kaempferol-3, 4′-di-<i>O</i>-β-D-glucoside, (3) 5, 7, 4′-trihydroxy-3′-methoxyflavone-3-<i>O</i>-β-D-sophoroside and (4) kaempferol-3-<i>O</i>-β-D-glucosyl-(2→l)-β-D-glucoside. This study will also provide useful reference standards for qualification and quantification of four flavonoid glycosides in natural products.</p></div>