10.6084/m9.figshare.1480973.v3 Yanxia Nie Yanxia Nie Li Li Li Li Mengcen Wang Mengcen Wang Teemu Tahvanainen Teemu Tahvanainen Yasuyuki Hashidoko Yasuyuki Hashidoko Nitrous oxide emission potentials of <i>Burkholderia</i> species isolated from the leaves of a boreal peat moss <i>Sphagnum fuscum</i><sup>*</sup> Taylor & Francis Group 2018 nitrous oxide (N2O) emitter Burkholderia Sphagnum moss polyphenols Sphagnum fuscum nosZ gene 2018-04-03 12:43:04 Dataset https://tandf.figshare.com/articles/dataset/Nitrous_oxide_emission_potentials_of_i_Burkholderia_i_species_isolated_from_the_leaves_of_a_boreal_peat_moss_i_Sphagnum_fuscum_i_a_href_FN0001_target_blank_a_/1480973 <p>Using a culture-based nitrous oxide (N<sub>2</sub>O) emission assay, three active N<sub>2</sub>O emitters were isolated from <i>Sphagnum fuscum</i> leaves and all identified as members of <i>Burkholderia</i>. These isolates showed N<sub>2</sub>O emission in the medium supplemented with but not with , and <i>Burkholderia</i> sp. SF-E2 showed the most efficient N<sub>2</sub>O emission (0.20 μg·vial<sup>−1</sup>·day<sup>−1</sup>) at 1.0 mM KNO<sub>3</sub>. In <i>Burkholderia</i> sp. SF-E2, the optimum pH for N<sub>2</sub>O production was 5.0, close to that of the phyllosphere of <i>Sphagnum</i> mosses, while the optimum temperature was uniquely over 30 °C. The stimulating effect of additional 1.5 mM sucrose on N<sub>2</sub>O emission was ignorable, but <i>Burkholderia</i> sp<i>.</i> SF-E2 upon exposure to 100 mg·L<sup>−1</sup> <i>E</i>-caffeic acid showed uniquely 67-fold higher N<sub>2</sub>O emission. All of the three N<sub>2</sub>O emitters were negative in both acetylene inhibition assay and PCR assay for <i>nosZ</i>-detection, suggesting that N<sub>2</sub>O reductase or the gene itself is missing in the N<sub>2</sub>O-emitting <i>Burkholderia</i>.</p> <p>Upon exposure to <i>E</i>-caffeic acid often contained in <i>Sphagnum</i> mosses, N<sub>2</sub>O emission by <i>Burkholderia</i> sp. SF-E2 that was isolated as an epiphyte of <i>Sphagnum fuscum</i> was highly accelerated.</p>