Simultaneous determination of seven bioactive components in Guizhi Fuling capsule by microwave-assisted extraction combined with ultra performance liquid chromatography tandem mass spectrometry
Yang Sui
Long-Shan Zhao
Zhen-zhong Wang
Wei Xiao
Zhi-Li Xiong
Yu-Tong Zhao
10.6084/m9.figshare.1488633.v1
https://tandf.figshare.com/articles/journal_contribution/Simultaneous_determination_of_seven_bioactive_components_in_Guizhi_Fuling_capsule_by_microwave_assisted_extraction_combined_with_ultra_performance_liquid_chromatography_tandem_mass_spectrometry/1488633
<div><p>A simple, rapid and reliable microwave-assisted extraction (MAE) combined with ultra performance liquid chromatography tandem mass spectrometry method was developed for simultaneous determination of the seven bioactive constituents in Guizhi Fuling capsule (GFC), namely gallic acid, amygdalin, albiflorin, paeoniflorin, paeonol, cinnamic acid and pachymic acid, respectively. The operation of MAE optimised through orthogonal array design experiment was performed at 80°C for 10 min with methanol–water (70:30, v/v) as the extracting solvent. The method was validated including intra- and inter-day precision, repeatability and stability, with relative standard deviation less than 3.9%, 3.3%, 4.4% and 3.1%, respectively. All analytes showed the good linearity (<i>r</i> >0.999), and their average recoveries varied between 98.2% and 101.2%. The results indicated that this method was simple, effective and suitable for the quality control of GFC.</p></div>
2015-07-20 00:00:00
quality control
bioactive constituents
Simultaneous determination
pachymic acid
extraction
performance
chromatography tandem mass spectrometry
gallic acid
chromatography tandem mass spectrometry method
GFC
MAE optimised
cinnamic acid
orthogonal array design experiment
bioactive components
Guizhi Fuling capsule