Common γ-chain cytokine signaling is required for macroautophagy induction during CD4<sup>+</sup> T-cell activation Yair Botbol Bindi Patel Fernando Macian 10.6084/m9.figshare.1569191 https://tandf.figshare.com/articles/dataset/Common_chain_cytokine_signaling_is_required_for_macroautophagy_induction_during_CD4_sup_sup_T_cell_activation/1569191 <p>Macroautophagy is a cellular process that mediates degradation in the lysosome of cytoplasmic components including proteins and organelles. Previous studies have shown that macroautophagy is induced in activated T cells to regulate organelle homeostasis and the cell's energy metabolism. However, the signaling pathways that initiate and regulate activation-induced macroautophagy in T cells have not been identified. Here, we show that activation-induced macroautophagy in T cells depends on signaling from common γ-chain cytokines. Consequently, inhibition of signaling through JAK3, induced downstream of cytokine receptors containing the common γ-chain, prevents full induction of macroautophagy in activated T cells. Moreover, we found that common γ-chain cytokines are not only required for macroautophagy upregulation during T cell activation but can themselves induce macroautophagy. Our data also show that macroautophagy induction in T cells is associated with an increase of LC3 expression that is mediated by a post-transcriptional mechanism. Overall, our findings unveiled a new role for common γ-chain cytokines as a molecular link between autophagy induction and T-cell activation.</p> 2015-10-30 17:44:02 common γ-chain interleukin 2 JAK macroautophagy T cell