L. Shyaula, Sajan Tamang, Tensangmu Ghouri, Nida Adhikari, Achyut Marasini, Shanti Bajracharya, Gan B. D. Manandhar, Mangala Choudhary, Muhammad Iqbal Antileishmanial diterpenoid alkaloids from <i>Aconitum spicatum</i> (Bruhl) Stapf <p>The crude extracts of tubers of <i>Aconitum spicatum</i> (Bruhl) Stapf were investigated for <i>in vitro</i> antileishmanial activity against <i>Leishmania major</i>. The dichloromethane extract at pH 2.5 showed antileishmanial activity with IC<sub>50</sub> value of 27.10 ± 0.0 μg/mL. Chromatographic purification of the dichloromethane extract led to isolation of three C-19 norditerpenoid alkaloids indaconitine (<b>1</b>), chasmaconitine (<b>2</b>) and ludaconitine (<b>3</b>). Compounds <b>3</b> and <b>2</b> showed antileishmanial activity with IC<sub>50</sub> = 36.10 ± 3.4 and 56.30 ± 2.1 μg/mL, respectively. Compound <b>1</b> was less effective (IC<sub>50</sub> > 100 μg/mL). The cytotoxicity of compounds <b>1</b>, <b>2</b> and <b>3</b> studied against MCF7, HeLa and PC3 cancer cell lines and 3T3 normal fibroblast cell line did not show cytotoxicity at 30 μM.</p> Aconitum spicatum;antileishmanial activity;Leishmania major;diterpenoid alkaloids 2015-11-28
    https://tandf.figshare.com/articles/journal_contribution/Antileishmanial_diterpenoid_alkaloids_from_i_Aconitum_spicatum_i_Bruhl_Stapf/1613547
10.6084/m9.figshare.1613547.v1