10.6084/m9.figshare.2068449 Yanqi Wu Yanqi Wu Darja Dobermann Darja Dobermann Michael H. Beale Michael H. Beale Jane L. Ward Jane L. Ward Acutifoliside, a novel benzoic acid glycoside from <i>Salix acutifolia</i> Taylor & Francis Group 2016 Salix acutifolia phenolic glycoside benzoic acid glycoside acutifoliside UHPLC–MS NMR 2016-01-28 23:39:00 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Acutifoliside_a_novel_benzoic_acid_glycoside_from_i_Salix_acutifolia_i_/2068449 <p>Ultra high-performance liquid chromatography–mass spectrometry (UHPLC–MS) profiling of a polar solvent extract of juvenile stem tissue of <i>Salix acutifolia</i> Willd. identified a range of phenolic metabolites. Salicortin, <b>1</b>, a well-known salicinoid, was the major compound present and the study identified young stem tissue of this species as a potential source of this compound for future studies. Three further known metabolites (salicin <b>2,</b> catechin <b>3</b> and tremuloidin <b>4</b>) were also present. The UHPLC–MS analysis also revealed the presence of a further, less polar, unknown compound, which was isolated via HPLC peak collection. The structure was elucidated by high-resolution mass spectroscopic analysis, 1- and 2-D NMR analysis and chemical derivatisation and was shown to be a novel benzoic acid glycoside <b>5</b>, which we have named as acutifoliside.</p>