%0 Generic %A Salvador-Recatalà, Vicenta %D 2016 %T New roles for the GLUTAMATE RECEPTOR-LIKE 3.3, 3.5, and 3.6 genes as on/off switches of wound-induced systemic electrical signals %U https://tandf.figshare.com/articles/dataset/New_roles_for_the_i_GLUTAMATE_RECEPTOR_LIKE_3_3_3_5_i_and_i_3_6_i_genes_as_on_off_switches_of_wound_induced_systemic_electrical_signals/3113017 %R 10.6084/m9.figshare.3113017 %2 https://tandf.figshare.com/ndownloader/files/4836982 %2 https://tandf.figshare.com/ndownloader/files/4836985 %K Action potential %K aphid %K arabidopsis %K electrical penetration graph %K glutamate receptor-like channel %K root %K wounding %K phloem %X

Wounding induces systemic potentials in Arabidopsis thaliana that can be abolished by concomitant suppression of the GLUTAMATE RECEPTOR-LIKE GLR3.3 and GLR3.6 genes. However, the roles of specific GLR channels to these potentials remain unclear. Here I applied the Electrical Penetration Graph (EPG) to study the contribution of three GLR channels to wound-induced, systemically propagated electrical potentials in Arabidopsis thaliana. In contrast to recordings made with conventional rigs for whole-plant electrophysiology, the EPG allows for the unambiguous distinction of the phloem-propagated action potential (AP) from the electrical activity outside of the phloem. The data reported here suggest that: (a) the transmission of wound-induced, phloem-propagated AP to neighbor leaves, requires expression of GLR3.3 or GLR3.6, whereas GLR3.5 prevents its transmission to non-neighbor leaves; (b) the generation of wound-induced electrical signals outside the phloem network depends on GLR3.6 expression; and (c) wound-induced systemic potentials initiated in the shoot are transmitted to the root in the adult plant, which suggests a role for these electrical signals in coordinating the plant defenses in the shoot and in the root. Here, I propose a model for wound-induced systemic electrical signals at the molecular, cellular and anatomical level. In this model, GLR3.3 and GLR3.6 function as on switches for the propagation of wound-induced potentials beyond the wounded leaf, while GLR3.5 functions as an off switch that prevents the propagation of wound-induced electrical potentials to distal, non-neighbor leaves.

%I Taylor & Francis