Tamano, Koichi Miura, Ai Further increased production of free fatty acids by overexpressing a predicted transketolase gene of the pentose phosphate pathway in <i>Aspergillus oryzae faaA</i> disruptant <p>Free fatty acids are useful as source materials for the production of biodiesel fuel and various chemicals such as pharmaceuticals and dietary supplements. Previously, we attained a 9.2-fold increase in free fatty acid productivity by disrupting a predicted acyl-CoA synthetase gene (<i>faaA</i>, AO090011000642) in <i>Aspergillus oryzae</i>. In this study, we achieved further increase in the productivity by overexpressing a predicted transketolase gene of the pentose phosphate pathway in the <i>faaA</i> disruptant. The <i>A. oryzae</i> genome is predicted to have three transketolase genes and overexpression of AO090023000345, one of the three genes, resulted in phenotypic change and further increase (corresponding to an increased production of 0.38 mmol/g dry cell weight) in free fatty acids at 1.4-fold compared to the <i>faaA</i> disruptant. Additionally, the biomass of hyphae increased at 1.2-fold by the overexpression. As a result, free fatty acid production yield per liter of liquid culture increased at 1.7-fold by the overexpression.</p> <p>The <i>A. oryzae ΔfaaA_tktOE</i> overexpressing a predicted transketolase gene in the pentose phosphate pathway increased FFA yield at 1.7-fold from the <i>ΔfaaA</i> and showed phenotypic change.</p> transketolase gene overexpression;pentose phosphate pathway;free fatty acid;productivity;Aspergillus oryzae faaA disruptant 2016-03-21
    https://tandf.figshare.com/articles/dataset/Further_increased_production_of_free_fatty_acids_by_overexpressing_a_predicted_transketolase_gene_of_the_pentose_phosphate_pathway_in_i_Aspergillus_oryzae_faaA_i_disruptant/3120682
10.6084/m9.figshare.3120682.v1