10.6084/m9.figshare.3408856.v1 Linda Bengtström Linda Bengtström Anna Kjerstine Rosenmai Anna Kjerstine Rosenmai Xenia Trier Xenia Trier Lisbeth Krüger Jensen Lisbeth Krüger Jensen Kit Granby Kit Granby Anne Marie Vinggaard Anne Marie Vinggaard Malcolm Driffield Malcolm Driffield Jens Højslev Petersen Jens Højslev Petersen Non-targeted screening for contaminants in paper and board food-contact materials using effect-directed analysis and accurate mass spectrometry Taylor & Francis Group 2016 Food packaging non-target analysis high-resolution mass spectrometry paper and board effect-directed analysis aryl hydrocarbon receptor activity 2016-06-01 17:00:06 Dataset https://tandf.figshare.com/articles/dataset/Non-targeted_screening_for_contaminants_in_paper_and_board_food-contact_materials_using_effect-directed_analysis_and_accurate_mass_spectrometry/3408856 <p>Due to large knowledge gaps in chemical composition and toxicological data for substances involved, paper and board food-contact materials (P&B FCM) have been emerging as a FCM type of particular concern for consumer safety. This study describes the development of a step-by-step strategy, including extraction, high-performance liquid chromatography (HPLC) fractionation, tentative identification of relevant substances and <i>in vitro</i> testing of selected tentatively identified substances. As a case study, we used two fractions from a recycled pizza box sample which exhibited aryl hydrocarbon receptor (AhR) activity. These fractions were analysed by gas chromatography (GC) and ultra-HPLC (UHPLC) coupled to quadrupole time-of-flight mass spectrometers (QTOF MS) in order tentatively to identify substances. The elemental composition was determined for peaks above a threshold, and compared with entries in a commercial mass spectral library for GC-MS (GC-EI-QTOF MS) analysis and an in-house built library of accurate masses for substances known to be used in P&B packaging for UHPLC-QTOF analysis. Of 75 tentatively identified substances, 15 were initially selected for further testing <i>in vitro</i>; however, only seven were commercially available and subsequently tested <i>in vitro</i> and quantified. Of these seven, the identities of three pigments found in printing inks were confirmed by UHPLC tandem mass spectrometry (QqQ MS/MS). Two pigments had entries in the database, meaning that a material relevant accurate mass database can provide a fast tentative identification. Pure standards of the seven tentatively identified substances were tested <i>in vitro</i> but could not explain a significant proportion of the AhR-response in the extract. Targeted analyses of dioxins and PCBs, both well-known AhR agonists, was performed. However, the dioxins could explain approximately 3% of the activity observed in the pizza box extract indicating that some very AhR active substance(s) still remain to be identified in recycled low quality P&B.</p>