10.6084/m9.figshare.3993669.v1 Sanga Mitra Sanga Mitra Arpa Samadder Arpa Samadder Pijush Das Pijush Das Smarajit Das Smarajit Das Medhanjali Dasgupta Medhanjali Dasgupta Jayprokas Chakrabarti Jayprokas Chakrabarti Decrypting ENCODEd epigenetic marks of human tRN-A-RS genes in normal, stem and cancer cell lines Taylor & Francis Group 2016 histone modifications chromatin state segmentation tRNA AARS RNA polymerase III RNA polymerase II 2016-10-06 19:19:16 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Decrypting_ENCODEd_epigenetic_marks_of_human_tRN-A-RS_genes_in_normal_stem_and_cancer_cell_lines/3993669 <p>Screening large-scale ENCODE data of 625 cytoplasmic transfer RNA (tRNAs) and 37 aminoacyl tRNA synthetase (AARSs) human genes, we deconstruct the array of relations between 10 histone marks affecting 15 chromatin states; their tissue specificity and variations and interchange amongst normal, cancerous and stem cells. The histone marks of RNA Pol II transcribed AARS genes share, but also contrast with that on RNA Pol III transcribed tRNA genes. tRNAs with identical/similar sequences may be in significantly varying states even within the same cell line; the chromatin scaffold, where the tRNA gene resides, is the key determinant. Hepatocellular carcinoma cell line has dominant H3K27me3, and singular clustering of other marks. Leukaemic cell line has hyperactive genes. The quiescence of the stem cells is encoded in the markers. Leaving aside the important exceptions in stem cells and elsewhere, tRNAs with cove scores above 50 have active markers and precise sets of transcription factors, and are usually well conserved compared to the low-scoring ones. Pseudo tRNAs are in heterochromatin/repressed state with anomalous exceptions in cancer cells. We motivate that Epigenetic-Phishing hacks the translation apparatus through the chromatin states governed by the histone marks of tRNA and AARS genes, and speculate on their therapeutic implications in cancer and on stem cells.</p>