10.6084/m9.figshare.4315823.v1 Aron Baksh Aron Baksh Bernhard Kepplinger Bernhard Kepplinger Hadiza A. Isah Hadiza A. Isah Michael R. Probert Michael R. Probert William Clegg William Clegg Corinne Wills Corinne Wills Michael Goodfellow Michael Goodfellow Jeff Errington Jeff Errington Nick Allenby Nick Allenby Michael J. Hall Michael J. Hall Production of 17-<i>O</i>-demethyl-geldanamycin, a cytotoxic ansamycin polyketide, by <i>Streptomyces hygroscopicus</i> DEM20745 Taylor & Francis Group 2016 17-O-demethyl-geldanamycin actinomycete polyketide ansamycin cytotoxic 2016-12-14 11:19:26 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Production_of_17-_i_O_i_-demethyl-geldanamycin_a_cytotoxic_ansamycin_polyketide_by_i_Streptomyces_hygroscopicus_i_DEM20745/4315823 <p>The actinomycete DEM20745, collected from non-rhizosphere soil adjacent to <i>Paraserianthes falactaria</i> trees (Cangkringan, Indonesia), is an efficient producer of the anticancer ansamycin polyketide 17-<i>O</i>-demethyl-geldanamycin (17-<i>O</i>-DMG), a biosynthetic precursor of the Hsp90 inhibitor geldanamycin (GDM). In DEM20745, 17-<i>O</i>-DMG is the major ansamycin product observed reaching a maximum titre of 17 mg/L in the fermentation broth. 17-<i>O</i>-DMG has the potential to be a key starting material for the semi-synthesis of GDM analogues for use in anticancer therapy. Thus, this preferential biosynthesis of 17-<i>O</i>-DMG facilitates easy access to this important molecule and provides further insight in the biosynthesis of the geldanamycins.</p>