10.6084/m9.figshare.4315823.v1
Aron Baksh
Aron
Baksh
Bernhard Kepplinger
Bernhard
Kepplinger
Hadiza A. Isah
Hadiza A.
Isah
Michael R. Probert
Michael R.
Probert
William Clegg
William
Clegg
Corinne Wills
Corinne
Wills
Michael Goodfellow
Michael
Goodfellow
Jeff Errington
Jeff
Errington
Nick Allenby
Nick
Allenby
Michael J. Hall
Michael J.
Hall
Production of 17-<i>O</i>-demethyl-geldanamycin, a cytotoxic ansamycin polyketide, by <i>Streptomyces hygroscopicus</i> DEM20745
Taylor & Francis Group
2016
17-O-demethyl-geldanamycin
actinomycete
polyketide
ansamycin
cytotoxic
2016-12-14 11:19:26
Journal contribution
https://tandf.figshare.com/articles/journal_contribution/Production_of_17-_i_O_i_-demethyl-geldanamycin_a_cytotoxic_ansamycin_polyketide_by_i_Streptomyces_hygroscopicus_i_DEM20745/4315823
<p>The actinomycete DEM20745, collected from non-rhizosphere soil adjacent to <i>Paraserianthes falactaria</i> trees (Cangkringan, Indonesia), is an efficient producer of the anticancer ansamycin polyketide 17-<i>O</i>-demethyl-geldanamycin (17-<i>O</i>-DMG), a biosynthetic precursor of the Hsp90 inhibitor geldanamycin (GDM). In DEM20745, 17-<i>O</i>-DMG is the major ansamycin product observed reaching a maximum titre of 17 mg/L in the fermentation broth. 17-<i>O</i>-DMG has the potential to be a key starting material for the semi-synthesis of GDM analogues for use in anticancer therapy. Thus, this preferential biosynthesis of 17-<i>O</i>-DMG facilitates easy access to this important molecule and provides further insight in the biosynthesis of the geldanamycins.</p>