Production of 17-<i>O</i>-demethyl-geldanamycin, a cytotoxic ansamycin polyketide, by <i>Streptomyces hygroscopicus</i> DEM20745
Aron Baksh
Bernhard Kepplinger
Hadiza A. Isah
Michael R. Probert
William Clegg
Corinne Wills
Michael Goodfellow
Jeff Errington
Nick Allenby
Michael J. Hall
10.6084/m9.figshare.4315823.v1
https://tandf.figshare.com/articles/journal_contribution/Production_of_17-_i_O_i_-demethyl-geldanamycin_a_cytotoxic_ansamycin_polyketide_by_i_Streptomyces_hygroscopicus_i_DEM20745/4315823
<p>The actinomycete DEM20745, collected from non-rhizosphere soil adjacent to <i>Paraserianthes falactaria</i> trees (Cangkringan, Indonesia), is an efficient producer of the anticancer ansamycin polyketide 17-<i>O</i>-demethyl-geldanamycin (17-<i>O</i>-DMG), a biosynthetic precursor of the Hsp90 inhibitor geldanamycin (GDM). In DEM20745, 17-<i>O</i>-DMG is the major ansamycin product observed reaching a maximum titre of 17 mg/L in the fermentation broth. 17-<i>O</i>-DMG has the potential to be a key starting material for the semi-synthesis of GDM analogues for use in anticancer therapy. Thus, this preferential biosynthesis of 17-<i>O</i>-DMG facilitates easy access to this important molecule and provides further insight in the biosynthesis of the geldanamycins.</p>
2016-12-14 11:19:26
17-O-demethyl-geldanamycin
actinomycete
polyketide
ansamycin
cytotoxic