Rinaldi, Federica Favero, Elena Del Rondelli, Valeria Pieretti, Stefano Bogni, Alessia Ponti, Jessica Rossi, François Marzio, Luisa Di Paolino, Donatella Marianecci, Carlotta Carafa, Maria pH-sensitive niosomes: Effects on cytotoxicity and on inflammation and pain in murine models <p>pH-sensitive nonionic surfactant vesicles (niosomes) by polysorbate-20 (Tween-20) or polysorbate-20 derivatized by glycine (added as pH sensitive agent), were developed to deliver Ibuprofen (IBU) and Lidocaine (LID). For the physical-chemical characterization of vesicles (mean size, size distribution, zeta potential, vesicle morphology, bilayer properties and stability) dynamic light scattering (DLS), small angle X-ray scattering and fluorescence studies were performed. Potential cytotoxicity was evaluated on immortalized human keratinocyte cells (HaCaT) and on immortalized mouse fibroblasts Balb/3T3. <i>In vivo</i> antinociceptive activity (formalin test) and anti-inflammatory activity tests (paw edema induced by zymosan) in murine models were performed on drug-loaded niosomes. pH-sensitive niosomes were stable in the presence of 0 and 10% fetal bovine serum, non-cytotoxic and able to modify IBU or LID pharmacological activity <i>in vivo</i>. The synthesis of stimuli responsive surfactant, as an alternative to add pH-sensitive molecules to niosomes, could represent a promising delivery strategy for anesthetic and anti-inflammatory drugs.</p> pH-sensitive niosomes;lidocaine;ibuprofen;cytotoxicity;anti-nociceptive/anti-inflammatory activity 2017-01-24
    https://tandf.figshare.com/articles/journal_contribution/pH-sensitive_niosomes_Effects_on_cytotoxicity_and_on_inflammation_and_pain_in_murine_models/4580524
10.6084/m9.figshare.4580524.v1