%0 Journal Article %A Kubica, Paweł %A Szopa, Agnieszka %A Ekiert, Halina %D 2017 %T Production of verbascoside and phenolic acids in biomass of Verbena officinalis L. (vervain) cultured under different in vitro conditions %U https://tandf.figshare.com/articles/journal_contribution/Production_of_verbascoside_and_phenolic_acids_in_biomass_of_i_Verbena_officinalis_i_L_vervain_cultured_under_different_i_in_vitro_i_conditions/4738429 %R 10.6084/m9.figshare.4738429.v1 %2 https://tandf.figshare.com/ndownloader/files/7742008 %K White light %K darkness %K plant growth regulators %K free phenolic acids %K bound phenolic acids %K HPLC-DAD analysis %X

Methanolic extracts from the biomass of Verbena officinalis cultured under continuous artificial light and in darkness on 12 variants of the Murashige and Skoog medium containing different concentrations (0.5–3.0 mg/L) of plant growth regulators: 6-benzyladenine, kinetin, 1-naphthaleneacetic acid and indole-3-butyric acid, were analysed for the amounts of verbascoside and phenolic acids, before and after acid hydrolysis, using the HPLC-DAD method. The amounts of verbascoside were very high (max. 2454.12 mg/100 g DW – light, and 2135.59 mg/100 g DW – darkness). The total amounts of phenolic acids reached a maximum of 46.02 mg/100 g DW (free phenolic acids) and 141.05 mg/100 g DW (bound compounds). The main metabolites were: ferulic, o-coumaric and caffeic acids. The maximum amount of verbascoside was 3.28 times higher than in extracts from the herb of the parent plant. The cultures could be proposed as a potential biotechnological source for selected biologically active compounds.

%I Taylor & Francis