10.6084/m9.figshare.5097556 MD. Ashraf Ashraf Javed Abrar Farooqi Javed Abrar Farooqi Kalim Javed Kalim Javed Evaluation of macrophage injury and activation by amphotericin B-loaded polymeric nanoparticles Taylor & Francis Group 2017 Amphotericin B drug delivery macrophage NIPAM phagocytosis NIPAM: N-isopropylacrylamide AA: acrylic acid VP: vinyl pyrrolidone AmB: amphotericin B MBA: N,N-methylene-bis-acrylamide ROS: reactive oxygen species SOD: superoxide dismutase DSC: differential scanning calorimeter TGA: thermogravimetric analysis DNA: deoxy ribonucleic acid IFN-γ, type II interferon TGF: transforming growth factor 2017-08-11 14:20:48 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Evaluation_of_Macrophage_Injury_and_Activation_by_Amphotericin_B_Loaded_Polymeric_Nanoparticles/5097556 <p>The objectives of present work were to prepare water soluble nanoformulation of amphotericin B by radical polymerization using <i>N</i>-isopropylacrylamide–acrylic acid and vinyl pyrrolidone for targeted delivery and minimized toxicity in mild experimental conditions with relatively narrow size distribution. The prepared formulations were extensively characterized in terms of size, morphology, thermal stability, drug loading and toxicity. The results demonstrates that the prepared nanoformulation have the potential to deliver amphotericin B with increased bioavailability and cellular uptake.</p>