10.6084/m9.figshare.5135023.v1 Li-Qing Deng Li-Qing Deng Si-Yu Zhou Si-Yu Zhou Jin-Xin Mao Jin-Xin Mao Shuang Liu Shuang Liu Xiao-Zhong Lan Xiao-Zhong Lan Zhi-Hua Liao Zhi-Hua Liao Min Chen Min Chen HPLC-ESI-MS/MS analysis of phenolics and <i>in vitro</i> antioxidant activity of <i>Epilobium angustifolium</i> L. Taylor & Francis Group 2017 Epilobium angustifolium L. antioxidant activity phenolics HPLC-ESI-MS/MS 2017-06-22 06:00:05 Journal contribution https://tandf.figshare.com/articles/journal_contribution/HPLC-ESI-MS_MS_analysis_of_phenolics_and_i_in_vitro_i_antioxidant_activity_of_i_Epilobium_angustifolium_i_L_/5135023 <p>The aerial parts of <i>Epilobium</i> plants are widely used as folk medicine and food around the world. The present study was aimed to investigate the antioxidant activities and active chemical constituents from <i>Epilobium angustifolium</i> L. The results revealed that the EtOAc extract, rich in phenolic compounds and flavonoids (16.81 ± 0.67 g GAE/100 g extract and 4.95 ± 0.21 g QE/100 g extract, respectively), possessed significantly antioxidant activities in reducing power, DPPH radical scavenging activity, ABTS radical scavenging activity and highly in inhibiting lipid peroxidation activity. Simultaneously, active fractions F to H from EtOAc extracts showing potent <i>in vitro</i> antioxidant activities also contained high content of total phenolic and flavonoid. Twenty-eight compounds were identified as phenolic compounds and flavonoids by LC-MS/MS. The results illustrate that the <i>E. angustifolium</i> L., which is rich in phenolics, could be used as a natural resource of antioxidant ingredient.</p>