10.6084/m9.figshare.5463511.v1 Li-Han Zhu Li-Han Zhu Tian-Hui Bao Tian-Hui Bao Yue Deng Yue Deng Hua Li Hua Li Li-Xia Chen Li-Xia Chen Constituents from <i><i>Apium graveolens</i></i> and their anti-inflammatory effects Taylor & Francis Group 2017 Apium graveolens phthalide glycoside megastigmane glycoside nitric oxide 2017-10-03 09:55:36 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Constituents_from_i_i_Apium_graveolens_i_i_and_their_anti-inflammatory_effects/5463511 <p>A phthalide glycoside, (3<i>R</i>, 4<i>R</i>)-4-<i>O</i>-<i>β</i>-D-glucopyranosyl-senkyunolide (<b>1</b>), and a megastigmane glycoside, (6<i>S</i>, 7<i>R</i>)-3-oxo-megastigma-4, 8-dien-7-<i>O</i>-<i>β</i>-D-glucoside (<b>2</b>), along with two known aglycones (<b>3–4</b>), were isolated from the 70% EtOH extract of fresh whole grass of <i>Apium graveolens</i> L. Their structures were elucidated by extensive spectroscopic analysis. All of these compounds were tested for their inhibitory effects on nitric oxide (NO) production in the RAW 264.7 macrophages. Among them, compounds <b>3</b> and <b>4</b> showed potent inhibitory activity against LPS-induced nitric oxide production in RAW 264.7 macrophages, with IC<sub>50</sub> values of 24.0 ± 2.1 μM and 28.6 ± 2.4 μM, respectively.</p>