10.6084/m9.figshare.6143504.v1 Fozia A. Adem Fozia A. Adem Victor Kuete Victor Kuete Armelle T. Mbaveng Armelle T. Mbaveng Matthias Heydenreich Matthias Heydenreich Andreas Koch Andreas Koch Albert Ndakala Albert Ndakala Beatrice Irungu Beatrice Irungu Abiy Yenesew Abiy Yenesew Thomas Efferth Thomas Efferth Cytotoxic flavonoids from two <i>Lonchocarpus</i> species Taylor & Francis Group 2018 Lonchocarpus bussei Lonchocarpus eriocalyx Leguminosae isoflavone pterocarpan cytotoxicity 2018-04-16 07:34:38 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Cytotoxic_flavonoids_from_two_i_Lonchocarpus_i_species/6143504 <p>A new isoflavone, 4′-prenyloxyvigvexin A (<b>1</b>) and a new pterocarpan, (6a<i>R</i>,11a<i>R</i>)-3,8-dimethoxybitucarpin B (<b>2</b>) were isolated from the leaves of <i>Lonchocarpus bussei</i> and the stem bark of <i>Lonchocarpus eriocalyx</i>, respectively. The extract of <i>L. bussei</i> also gave four known isoflavones, maximaisoflavone H, 7,2′-dimethoxy-3′,4′-methylenedioxyisoflavone, 6,7,3′-trimethoxy-4′,5′-methylenedioxyisoflavone, durmillone; a chalcone, 4-hydroxylonchocarpin; a geranylated phenylpropanol, colenemol; and two known pterocarpans, (6a<i>R</i>,11a<i>R</i>)-maackiain and (6a<i>R</i>,11a<i>R</i>)-edunol. (6a<i>R</i>,11a<i>R</i>)-Edunol was also isolated from the stem bark of <i>L. eriocalyx</i>. The structures of the isolated compounds were elucidated by spectroscopy. The cytotoxicity of the compounds was tested by resazurin assay using drug-sensitive and multidrug-resistant cancer cell lines. Significant antiproliferative effects with IC<sub>50</sub> values below 10 μM were observed for the isoflavones 6,7,3′-trimethoxy-4′,5′-methylenedioxyisoflavone and durmillone against leukemia CCRF-CEM cells; for the chalcone, 4-hydroxylonchocarpin and durmillone against its resistant counterpart CEM/ADR5000 cells; as well as for durmillone against the resistant breast adenocarcinoma MDA-MB231/<i>BCRP</i> cells and resistant gliobastoma U87MG<i>.</i>Δ<i>EGFR</i> cells.</p>