10.6084/m9.figshare.6143504.v1
Fozia A. Adem
Fozia A.
Adem
Victor Kuete
Victor
Kuete
Armelle T. Mbaveng
Armelle T.
Mbaveng
Matthias Heydenreich
Matthias
Heydenreich
Andreas Koch
Andreas
Koch
Albert Ndakala
Albert
Ndakala
Beatrice Irungu
Beatrice
Irungu
Abiy Yenesew
Abiy
Yenesew
Thomas Efferth
Thomas
Efferth
Cytotoxic flavonoids from two <i>Lonchocarpus</i> species
Taylor & Francis Group
2018
Lonchocarpus bussei
Lonchocarpus eriocalyx
Leguminosae
isoflavone
pterocarpan
cytotoxicity
2018-04-16 07:34:38
Journal contribution
https://tandf.figshare.com/articles/journal_contribution/Cytotoxic_flavonoids_from_two_i_Lonchocarpus_i_species/6143504
<p>A new isoflavone, 4′-prenyloxyvigvexin A (<b>1</b>) and a new pterocarpan, (6a<i>R</i>,11a<i>R</i>)-3,8-dimethoxybitucarpin B (<b>2</b>) were isolated from the leaves of <i>Lonchocarpus bussei</i> and the stem bark of <i>Lonchocarpus eriocalyx</i>, respectively. The extract of <i>L. bussei</i> also gave four known isoflavones, maximaisoflavone H, 7,2′-dimethoxy-3′,4′-methylenedioxyisoflavone, 6,7,3′-trimethoxy-4′,5′-methylenedioxyisoflavone, durmillone; a chalcone, 4-hydroxylonchocarpin; a geranylated phenylpropanol, colenemol; and two known pterocarpans, (6a<i>R</i>,11a<i>R</i>)-maackiain and (6a<i>R</i>,11a<i>R</i>)-edunol. (6a<i>R</i>,11a<i>R</i>)-Edunol was also isolated from the stem bark of <i>L. eriocalyx</i>. The structures of the isolated compounds were elucidated by spectroscopy. The cytotoxicity of the compounds was tested by resazurin assay using drug-sensitive and multidrug-resistant cancer cell lines. Significant antiproliferative effects with IC<sub>50</sub> values below 10 μM were observed for the isoflavones 6,7,3′-trimethoxy-4′,5′-methylenedioxyisoflavone and durmillone against leukemia CCRF-CEM cells; for the chalcone, 4-hydroxylonchocarpin and durmillone against its resistant counterpart CEM/ADR5000 cells; as well as for durmillone against the resistant breast adenocarcinoma MDA-MB231/<i>BCRP</i> cells and resistant gliobastoma U87MG<i>.</i>Δ<i>EGFR</i> cells.</p>