Interaction of S-layer proteins of <i>Lactobacillus kefir</i> with model membranes and cells Axel Hollmann Lucrecia Delfederico Nuno C. Santos E. Anibal Disalvo Liliana Semorile 10.6084/m9.figshare.6180734.v1 https://tandf.figshare.com/articles/journal_contribution/Interaction_of_S-layer_proteins_of_i_Lactobacillus_kefir_i_with_model_membranes_and_cells/6180734 <p>In previous works, it was shown that S-layer proteins from <i>Lactobacillus kefir</i> were able to recrystallize and stabilize liposomes, this feature reveling a great potential for developing liposomal-based carriers. Despite previous studies on this subject are important milestones, a number of questions remain unanswered. In this context, the feasibility of S-layer proteins as a biomaterial for drug delivery was evaluated in this work. First, S-layer proteins were fully characterized by electron microscopy, 2D-electrophoresis, and anionic exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD). Afterward, interactions of S-layer proteins with model lipid membranes were evaluated, showing that proteins adsorb to the lipid surface following a non-fickean or anomalous diffusion, when positively charged lipid were employed, suggesting that electrostatic interaction is a key factor in the recrystallization process on these proteins. Finally, the interaction of S-layer coated liposomes with Caco-2 cell line was assessed: First, cytotoxicity of formulations was tested showing no cytotoxic effects in S-layer coated vesicles. Second, by flow cytometry, it was observed an increased ability to transfer cargo molecules into Caco-2 cells from S-layer coated liposomes in comparison to control ones. All data put together, supports the idea that a combination of adhesive properties of S-layer proteins concomitant with higher stability of S-layer coated liposomes represents an exciting starting point in the development of new drug carriers.</p> 2018-04-25 06:20:58 transfer cargo molecules Caco -2 cell line liposomes HPAEC-PAD Lactobacillus kefir model lipid membranes Caco -2 cells S-layer proteins