%0 Journal Article %A Hollmann, Axel %A Delfederico, Lucrecia %A Santos, Nuno C. %A Disalvo, E. Anibal %A Semorile, Liliana %D 2018 %T Interaction of S-layer proteins of Lactobacillus kefir with model membranes and cells %U https://tandf.figshare.com/articles/journal_contribution/Interaction_of_S-layer_proteins_of_i_Lactobacillus_kefir_i_with_model_membranes_and_cells/6180734 %R 10.6084/m9.figshare.6180734.v1 %2 https://tandf.figshare.com/ndownloader/files/11194223 %K transfer cargo molecules %K Caco -2 cell line %K liposomes %K HPAEC-PAD %K Lactobacillus kefir %K model lipid membranes %K Caco -2 cells %K S-layer proteins %X

In previous works, it was shown that S-layer proteins from Lactobacillus kefir were able to recrystallize and stabilize liposomes, this feature reveling a great potential for developing liposomal-based carriers. Despite previous studies on this subject are important milestones, a number of questions remain unanswered. In this context, the feasibility of S-layer proteins as a biomaterial for drug delivery was evaluated in this work. First, S-layer proteins were fully characterized by electron microscopy, 2D-electrophoresis, and anionic exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD). Afterward, interactions of S-layer proteins with model lipid membranes were evaluated, showing that proteins adsorb to the lipid surface following a non-fickean or anomalous diffusion, when positively charged lipid were employed, suggesting that electrostatic interaction is a key factor in the recrystallization process on these proteins. Finally, the interaction of S-layer coated liposomes with Caco-2 cell line was assessed: First, cytotoxicity of formulations was tested showing no cytotoxic effects in S-layer coated vesicles. Second, by flow cytometry, it was observed an increased ability to transfer cargo molecules into Caco-2 cells from S-layer coated liposomes in comparison to control ones. All data put together, supports the idea that a combination of adhesive properties of S-layer proteins concomitant with higher stability of S-layer coated liposomes represents an exciting starting point in the development of new drug carriers.

%I Taylor & Francis