Kasanah, Noer Amelia, Windi Mukminin, Amirul Triyanto Isnansetyo, Alim Antibacterial activity of Indonesian red algae <i>Gracilaria edulis</i> against bacterial fish pathogens and characterization of active fractions <p>In this study the crude ethyl acetate extracts and fractions from red algae <i>Gracilaria edulis</i> were studied against fish pathogens. <i>G. edulis</i> was extracted with ethyl acetate, fractionated in silica gel coloum chromatography and gradient hexane-ethyl acetate. The extract and fractions were subjected for bioassay guided isolation against fish pathogen <i>Vibrio</i> spp and <i>Aeromonas hydrophila</i>. Bioassays were accomplished by diffusion agar, bioauthography, and 96 well microplates format. Profiling chemical constituent was done by thin layer chromatography and GC-MS. Metabolites analysis using GC-MS yielded chemical constituents of active fractions as 9-hexadecenoic acid, hexadecanoic acid, 13-octadecenoic acid, 10-octadecenoic acid, eicosanoic acid, cholest-8-en-3-ol. The results provide <i>in vitro</i> scientific support to the possible application of <i>Gracilaria edulis</i> as antimicrobial agent that may contribute for treatment of bacterial infections in aquaculture.</p> Gracilaria edulis;Aeromonas hydrophila;Vibrio spp;GC-MS;fatty acid;sterol 2018-05-07
    https://tandf.figshare.com/articles/journal_contribution/Antibacterial_activity_of_Indonesian_red_algae_i_Gracilaria_edulis_i_against_bacterial_fish_pathogens_and_characterization_of_active_fractions/6228902
10.6084/m9.figshare.6228902.v1