10.6084/m9.figshare.6231716.v1
David Lin
David
Lin
Jonathan Li
Jonathan
Li
Rabail Razi
Rabail
Razi
Niha Qamar
Niha
Qamar
Laurie Levine
Laurie
Levine
Thomas Zimmerman
Thomas
Zimmerman
Sayyed A. Hamidi
Sayyed
A. Hamidi
Millicent Schmidt
Millicent
Schmidt
Marc G. Golightly
Marc G.
Golightly
Todd Rueb
Todd
Rueb
Andrea Harrington
Andrea
Harrington
Merrill Garnett
Merrill
Garnett
Frank Antonawich
Frank
Antonawich
Steven McClain
Steven
McClain
Edmund Miller
Edmund
Miller
Courtney Cox
Courtney
Cox
Po Hsuan Huang
Po Hsuan
Huang
Anthony M. Szema
Anthony
M. Szema
Rux largely restores lungs in Iraq PM-exposed mice, Up-regulating regulatory T-cells (Tregs)
Taylor & Francis Group
2018
Burn pits
dust
fibrosis
iraq
inflammation
lung injury
mice
particulate matter
rux
2018-05-08 15:49:15
Dataset
https://tandf.figshare.com/articles/dataset/Rux_largely_restores_lungs_in_Iraq_PM-exposed_mice_Up-regulating_regulatory_T-cells_Tregs_/6231716
<p><b>Background</b> Military personnel post-deployment to Iraq and Afghanistan have noted new-onset respiratory illness. This study's primary objective was to further develop an animal model of Iraq Afghanistan War Lung Injury (IAW-LI) and to test a novel class of anti-injury drug called RuX. <b>Methods</b> Particulate Matter (PM) samples were obtained in Iraq then characterized by spectromicroscopy. C57BL/6 mice underwent orotracheal instillation with PM, followed by drinkable treatment with RuX. Lung histology, inspiratory capacity (FlexiVent), thymic/splenic regulatory T cell (Treg) number, and whole-lung genomics were analyzed. <b>Results</b> Tracheal instillation of Iraq PM led to lung septate thickening and lymphocytic inflammation. PM-exposed mice had suppression of thymic/splenic regulatory T-cells (Tregs). Drinking RuX after PM exposure attenuated the histologic lung injury response, improved lung inspiratory capacity, and increased Tregs. Pooled whole lung genomics suggest differences among gene expression of IL-15 among control, PM, and PM + RuX groups. <b>Conclusions</b> RuX, a ruthenium and alpha-lipoic acid complex, attenuates lung injury by improving histology and inspiratory capacity via upregulation of Tregs in Iraq PM-exposed C57BL/6. Plausible genomic effects may involve IL-15 whole lung gene expression.</p>