%0 Generic %A Lu, Cuiling %A Chi, Hongbin %A Wang, Yapeng %A Feng, Xue %A Wang, Lina %A Huang, Shuo %A Yan, Liying %A Lin, Shengli %A Liu, Ping %A Qiao, Jie %D 2018 %T Transcriptome analysis of PCOS arrested 2-cell embryos %U https://tandf.figshare.com/articles/dataset/Transcriptome_analysis_of_PCOS_arrested_2-cell_embryos/6587396 %R 10.6084/m9.figshare.6587396.v1 %2 https://tandf.figshare.com/ndownloader/files/12074450 %2 https://tandf.figshare.com/ndownloader/files/12074453 %2 https://tandf.figshare.com/ndownloader/files/12074456 %2 https://tandf.figshare.com/ndownloader/files/12074462 %2 https://tandf.figshare.com/ndownloader/files/12074465 %2 https://tandf.figshare.com/ndownloader/files/12074468 %2 https://tandf.figshare.com/ndownloader/files/12074471 %2 https://tandf.figshare.com/ndownloader/files/12074474 %2 https://tandf.figshare.com/ndownloader/files/12074477 %K PCOS %K embryo arrest %K transcriptome %X

In an attempt to explore the early developmental arrest in embryos from polycystic ovarian syndrome (PCOS) patients, we sequenced the transcriptome profiles of PCOS arrested 2-cell embryos, non-PCOS arrested 2-cell embryos and non-arrested 2-cell embryos using single-cell RNA-Seq technique. Differential expression analysis was performed using the DEGSeq R package. Gene Ontology (GO) enrichment was analyzed using the GOseq R package. Data revealed 62 differentially expressed genes between non-PCOS arrested and PCOS arrested embryos and 2217 differentially expressed genes between PCOS arrested and non-arrested 2-cell embryos. A total of 49 differently expressed genes (DEGs) were annotated with GO terms in the up-regulated genes between PCOS arrested and non-PCOS arrested embryos after GO enrichment. A total of 29 DEGs were annotated with GO terms in the down-regulated genes between PCOS arrested and non-arrested 2-cell embryos after GO enrichment. These data can provide a reference for screening specific genes involved in the arrest of PCOS embryos.

%I Taylor & Francis