10.6084/m9.figshare.6886358.v1 Qing Xu Qing Xu Edwin C. M. Mariman Edwin C. M. Mariman Nadia J. T. Roumans Nadia J. T. Roumans Roel G. Vink Roel G. Vink Gijs H. Goossens Gijs H. Goossens Ellen E. Blaak Ellen E. Blaak Johan W. E. Jocken Johan W. E. Jocken Adipose tissue autophagy related gene expression is associated with glucometabolic status in human obesity Taylor & Francis Group 2018 Adipose tissue adipocyte autophagy lipolysis obesity glucometabolic status 2018-08-01 07:21:47 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Adipose_tissue_autophagy_related_gene_expression_is_associated_with_glucometabolic_status_in_human_obesity/6886358 <p>Adipose tissue autophagy (AT) is associated with human obesity and increased metabolic risk. Recent findings establish a role for autophagy in lipid metabolism. Here, we compared the expression of autophagy-related and lipolysis genes in human abdominal subcutaneous AT (SCAT) in overweight/obese subjects (n = 17) with or without impaired glucose tolerance in comparison with lean normal glucose tolerant individuals (n = 9), and investigated the association between AT autophagy and lipolysis. Human multipotent adipose-derived stem cells (hMADS) were used to investigate the effect of pharmacological HSL inhibition on changes in the autophagic flux. The expression of autophagy-related genes (ATG) 5, 7 and 12 in SCAT was significantly higher (p = 0.043, p = 0.015, p = 0.004, respectively) in overweight/obese compared to lean men, while expression of the classical lipases HSL (p = 0.092) and ATGL (p = 0.084) tended to be lower. ATG12 mRNA was positively correlated with BMI (r = 0.407, p = 0.039). ATG7 mRNA correlated positively with waist/hip ratio (WHR) (r = 0.420, p = 0.041), 2 h glucose concentration (r = 0.488, p = 0.011) and insulin (r = 0.419, p = 0.033). Multiple linear regressions revealed that ATG7 gene expression was positively related to 2 h glucose, independent of BMI, WHR and insulin. Gene expression interaction analysis showed that ATG7 mRNA negatively correlated with HSL (p<0.01) and ATGL mRNA expression (p<0.01). In line, treatment of differentiated hMADS with an HSL inhibitor increased LC3 accumulation, a marker of increased autophagic flux. Collectively, the present study demonstrated that a low expression of classical lipases in abdominal SCAT is accompanied by an increased expression of ATGs in overweight/obese subjects, which seems to be mainly related to glucose tolerance.</p>