10.6084/m9.figshare.7138094.v1 Mitra Tohidi Mitra Tohidi Majid Ramezani Majid Ramezani Ali Mehramizi Ali Mehramizi Response surface methodology for optimization and determination of Riluzole by microfunnel magnetic stirring-assisted liquid–liquid microextraction coupled with high-performance liquid chromatography Taylor & Francis Group 2018 Microfunnel magnetic stirring-assisted liquid–liquid microextraction Riluzole (RLZ) high-performance liquid chromatography water human plasma urine samples response surface methodology 2018-09-26 22:52:28 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Response_surface_methodology_for_optimization_and_determination_of_Riluzole_by_microfunnel_magnetic_stirring-assisted_liquid_liquid_microextraction_coupled_with_high-performance_liquid_chromatography/7138094 <p>A rapid, simple, and highly sensitive microfunnel magnetic stirring-assisted liquid–liquid microextraction coupled with HPLC was developed for determination of Riluzole (RLZ). The main extraction parameters were screened using the Plackett–Burman design and optimized by central composite design. Under optimum conditions, the calibration curve was linear over the range of 0.2–400 ng mL<sup>−1</sup>, and RSD of 3.21% (<i>n</i> = 14, 20 ng mL<sup>−1</sup>) proved to have good reproducibility. The detection limit was 0.023 ng mL<sup>−1</sup> and 1.5 ng mL<sup>−1</sup> for aqueous and biological samples, respectively. The tap water, human plasma, and urine samples showed excellent recoveries for RLZ (97.09–101.92%) via this method.</p>