Overexpression of MinE gene affects the plastid division in cassava
Cong-Cong Wang
Lei Ke
Liang-Jing Cao
Yuan Yao
Meng-Ting Geng
Ying Wang
Yu Xiao
Wu Huang
Xiao-Han Liu
Peng Cao
Jian-Chun Guo
Yi Min
10.6084/m9.figshare.7139600.v1
https://tandf.figshare.com/articles/journal_contribution/Overexpression_of_MinE_gene_affects_the_plastid_division_in_cassava/7139600
<p>The MinE protein plays an important role in plastid division. In this study, the <i>MinE</i> gene was isolated from the cassava (<i>Manihot esculenta</i> Crantz) genome. We isolated high quality and quantity protoplasts and succeed in performing the transient expression of the GFP-fused <i>Manihot esculenta</i> MinE (MeMinE) protein in cassava mesophyll protoplasts. The transient expression of <i>MeMinE-GFP</i> in cassava protoplasts showed that the MeMinE protein was located in the chloroplast. Due to the abnormal division of chloroplasts, overexpression of MeMinE proteins in cassava mesophyll protoplasts could result in fewer and smaller chloroplasts. Overexpression of MeMinE proteins also showed abnormal cell division characteristics and minicell occurrence in <i>Escherichia coli</i> caused by aberrant septation events in the cell poles.</p> <p>MeMinE proteins function in cassava chloroplasts and <i>Escherichia coli</i>. The MinE protein plays an important role in plastid division.</p>
2018-09-27 07:58:15
MeMinE gene
plastid division
protoplast isolation
subcellular localization
prokaryotic expression