10.6084/m9.figshare.7139639.v1 Suzuki Setsuko Suzuki Setsuko Kyoko Sugai Kyoko Sugai Kentaro Uchiyama Kentaro Uchiyama Saeko Katoh Saeko Katoh Hidetoshi Kato Hidetoshi Kato Satoshi Narita Satoshi Narita Yuji Isagi Yuji Isagi Development of microsatellite markers for <i>Callicarpa subpubescens</i> (Lamiaceae), an endemic species of the Bonin Islands Taylor & Francis Group 2018 Ecotype next-generation sequencing reproductive isolation RNA-seq simple sequence repeat markers 2018-09-27 08:01:23 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Development_of_microsatellite_markers_for_i_Callicarpa_subpubescens_i_Lamiaceae_an_endemic_species_of_the_Bonin_Islands/7139639 <p>Expressed sequence tagged (EST) microsatellite (SSR) markers were newly developed for <i>Callicarpa subpubescens</i>, a species endemic to the Bonin (Ogasawara) Islands of Japan, to investigate gene flow, genetic structure, and evolution of the species. A total of 87,334 contigs derived from <i>de novo</i> sequencing were obtained from which 1,826 primers bordering candidate SSRs were designed. Success of amplification and presence of polymorphisms were tested for each primer pair using two populations of <i>C. subpubescens</i>, and one population of <i>C. parvifolia</i> closely related to <i>C. subpubescens</i> to check cross-species amplification. Twenty-nine of the 48 primer pairs showed clear amplification, with 27 showing polymorphisms in the tested populations. For the 27 polymorphic loci, across the two species, the number of alleles ranged from two to 23 (average = 5.93), and expected heterozygosity per locus were 0.05–0.91 (average = 0.52). Bayesian clustering analysis for <i>C. subpubescens</i> showed that the most likely number of clusters is two, with each population dominated by a unique cluster. These new EST-SSR markers presented here can be used for population genetic study of <i>C. subpubescens</i> and its congener <i>C. parvifolia</i>.</p>