<i>In vitro</i> anti-inflammatory and free radical scavenging properties of <i>Oldenlandia auricularia</i> G. I. Anuja V. J. Shine S. R. Suja 10.6084/m9.figshare.7594154.v1 https://tandf.figshare.com/articles/dataset/_i_In_vitro_i_anti-inflammatory_and_free_radical_scavenging_properties_of_i_Oldenlandia_auricularia_i_/7594154 <p>The <i>in vitro</i> anti-inflammatory and antioxidant properties of extract of <i>Oldenlandia auricularia with</i> 70% aqueous alcohol have been evaluated. The inhibition of nuclear translocation of nuclear factor kappa-light-chain-enhancer of activated B cells in lipopolysaccharide stimulated murine macrophage cells was evaluated. Messenger ribonucleic acid levels of tumor necrosis factor-α and cyclooxygenase-2 were analysed by quantitative real time polymerase chain reaction. <i>O. auricularia</i> (100 mg/L) showed better anti-inflammatory and antioxidant activities when compared to its lower doses, whereas the standard drugs curcumin and diclofenac sodium showed significant anti-inflammatory and antioxidant potential. Treatment at this concentration inhibited the signalling pathway of nuclear factor kappa-light-chain-enhancer of activated B cells and messenger ribonucleic acid expression of tumor necrosis factor- α, and cyclooxygenase-2. Liquid chromatography/quadrupole time-of-flight mass spectrometry revealed the presence of previously reported compounds auricularine, ursolic acid and β-sitosterol. The observed anti-inflammatory property of <i>O. auricularia</i> could be due to the inhibition of nuclear factor kappa-light-chain-enhancer of activated B cells p65 sub unit nuclear translocation and inhibition of its signalling pathway.</p> 2019-01-16 13:10:41 Oldenlandia auricularia nuclear factor kappa-light-chain-enhancer B cells nuclear translocation tumour necrosis factor-α cyclooxygenase-2