%0 Generic %A Anuja, G. I. %A Shine, V. J. %A Suja, S. R. %D 2019 %T In vitro anti-inflammatory and free radical scavenging properties of Oldenlandia auricularia %U https://tandf.figshare.com/articles/dataset/_i_In_vitro_i_anti-inflammatory_and_free_radical_scavenging_properties_of_i_Oldenlandia_auricularia_i_/7594154 %R 10.6084/m9.figshare.7594154.v1 %2 https://tandf.figshare.com/ndownloader/files/14100722 %2 https://tandf.figshare.com/ndownloader/files/14100725 %K Oldenlandia auricularia %K nuclear factor kappa-light-chain-enhancer B cells %K nuclear translocation %K tumour necrosis factor-α %K cyclooxygenase-2 %X

The in vitro anti-inflammatory and antioxidant properties of extract of Oldenlandia auricularia with 70% aqueous alcohol have been evaluated. The inhibition of nuclear translocation of nuclear factor kappa-light-chain-enhancer of activated B cells in lipopolysaccharide stimulated murine macrophage cells was evaluated. Messenger ribonucleic acid levels of tumor necrosis factor-α and cyclooxygenase-2 were analysed by quantitative real time polymerase chain reaction. O. auricularia (100 mg/L) showed better anti-inflammatory and antioxidant activities when compared to its lower doses, whereas the standard drugs curcumin and diclofenac sodium showed significant anti-inflammatory and antioxidant potential. Treatment at this concentration inhibited the signalling pathway of nuclear factor kappa-light-chain-enhancer of activated B cells and messenger ribonucleic acid expression of tumor necrosis factor- α, and cyclooxygenase-2. Liquid chromatography/quadrupole time-of-flight mass spectrometry revealed the presence of previously reported compounds auricularine, ursolic acid and β-sitosterol. The observed anti-inflammatory property of O. auricularia could be due to the inhibition of nuclear factor kappa-light-chain-enhancer of activated B cells p65 sub unit nuclear translocation and inhibition of its signalling pathway.

%I Taylor & Francis