Deciphering foot-and-mouth disease (FMD) virus–host tropism
Indra Singh
Rajib Deb
Sanjeev Kumar
Rani Singh
Jerome Andonissamy
Shuchi Smita
Gyanendra Singh Sengar
Rajiv Kumar
Krishna Kumar Ojha
Nihar Ranjan Sahoo
S Murali
Rejani Chandran
Radhakrishnan V Nair
S B Lal
Dwijesh Chandra Mishra
Anil Rai
10.6084/m9.figshare.7604708.v2
https://tandf.figshare.com/articles/dataset/Deciphering_Foot-and-Mouth_Disease_FMD_virus-host_Tropism/7604708
<p>The pattern of interactions between foot and mouth disease (FMD) viral protein 1 (VP1) with susceptible and resistant host integrins were deciphered. The putative effect of site-directed mutation on alteration of interaction is illustrated using predicted and validated 3D structures of VP1, mutated VP1 and integrins of <i>Bos taurus</i>, <i>Gallus</i> and <i>Canis</i>. Strong interactions were observed between FMDV-VP1 protein motifs at conserved tripeptide, Arg-Gly-Asp <sup>143</sup>RGD<sup>145</sup> and at domain <sup>676</sup>SIPLQ<sup>680</sup> in alpha-integrin of <i>B. taurus.</i> Notably, <i>in-silico</i> site-directed mutation in FMDV-VP1 protein led to complete loss of interaction between FMD-VP1 protein and <i>B. taurus</i> integrin, which confirmed the active role of arginine-glycine-aspartic acid (RGD) domain. Interestingly, <i>in-vitro</i> analysis demonstrates the persistence of the putative tropism site ‘SIPLQ’ in different cattle breeds undertaken. Thus, the attempt to decipher the tropism of FMDV at host receptor level interaction might be useful for future FMD control strategies through development of mimetic marker vaccines and/or host receptor manipulations.</p> <p>Communicated by Ramaswamy H. Sarma</p>
2019-02-07 12:47:53
Foot-and-mouth disease (FMD)
integrin
interaction
KR779877
tropism