Liu, Yue Xu, Songhua Lian, Xiuli Su, Yang Zhong, Yuhuan Lv, Ruimin Mo, Kaien Zhu, Huimin Xiaojiang, Wang Xu, Lixuan Wang, Shie Atypical GATA protein TRPS1 plays indispensable roles in mouse two-cell embryo <p>Zygotic genome activation (ZGA) is one of the most critical events at the beginning of mammalian preimplantation embryo development (PED). The mechanisms underlying mouse ZGA remain unclear although it has been widely studied. In the present study, we identified that tricho-rhino-phalangeal syndrome 1 (TRPS1), an atypical GATA family member, is an important factor for ZGA in mouse PED. We found that the <i>Trps1</i> mRNA level peaked at the one-cell stage while TRPS1 protein did so at the two/four-cell stage. Knockdown of <i>Trps1</i> by the microinjection of <i>Trps1</i> siRNA reduced the developmental rate of mouse preimplantation embryos by approximately 30%, and increased the expression of ZGA marker genes <i>MuERV-L</i> and <i>Zscan4d</i> via suppressing the expression of major histone markers H3K4me3 and H3K27me3. Furthermore, <i>Trps1</i> knockdown decreased the expression of <i>Sox2</i> but increased <i>Oct4</i> expression. We conclude that TRPS1 may be indispensable for zygotic genome activation during mouse PED.</p> Zygotic genome activation (ZGA);preimplantation embryo development (PED);tricho-rhino-phalangeal syndrome 1 (TRPS1) 2019-02-12
    https://tandf.figshare.com/articles/journal_contribution/Atypical_GATA_protein_TRPS1_plays_indispensable_roles_in_mouse_two-cell_embryo/7665374
10.6084/m9.figshare.7665374.v2