Biofilm inhibition and anti-quorum sensing activity of phytosynthesized silver nanoparticles against the nosocomial pathogen <i>Pseudomonas aeruginosa</i> Saloni Shah Swapnil Gaikwad Shuchi Nagar Shatavari Kulshrestha Viniti Vaidya Neelu Nawani Sarika Pawar 10.6084/m9.figshare.7687232.v1 https://tandf.figshare.com/articles/dataset/Biofilm_inhibition_and_anti-quorum_sensing_activity_of_phytosynthesized_silver_nanoparticles_against_the_nosocomial_pathogen_i_Pseudomonas_aeruginosa_i_/7687232 <p>Quorum sensing (QS), the communication signaling network, regulates biofilm formation and several virulence factors in <i>Pseudomonas aeruginosa</i> PAO1, a nosocomial opportunistic pathogen. QS is considered to be a challenging target for compounds antagonistic to virulent factors. Biologically synthesized silver nanoparticles (AgNPs) are reported as anti-QS and anti-biofilm drugs against bacterial infections. The present study reports on the synthesis and characterization of <i>Piper betle</i> (Pb) mediated AgNPs (Pb-AgNPs). The anti-QS activity of Pb-AgNPs against <i>Chromobacterium violaceum</i> and the potential effect of Pb-AgNPs on QS-regulated phenotypes in PAO1 were studied. FTIR analysis exhibited that Pb-AgNPs had been capped by phytochemical constituents of Pb. Eugenol is one of the active phenolic phytochemicals in Pb leaves, therefore molecular docking of eugenol-conjugated AgNPs on QS regulator proteins (LasR, LasI and MvfR) was performed. Eugenol-conjugated AgNPs showed considerable binding interactions with QS-associated proteins. These results provide novel insights into the development of phytochemically conjugated nanoparticles as promising anti-infective candidates.</p> 2019-02-07 08:13:31 Piper betle silver nanoparticles anti‒quorum sensing biofilm inhibition Pseudomonas aeruginosa PAO1 molecular docking