10.6084/m9.figshare.7872008.v3
Abubakar Wani
Abubakar
Wani
Mehak Gupta
Mehak
Gupta
Masroor Ahmad
Masroor
Ahmad
Aabid M. Shah
Aabid M.
Shah
Aitizaz Ul Ahsan
Aitizaz Ul
Ahsan
Parvaiz H Qazi
Parvaiz H
Qazi
Fayaz Malik
Fayaz
Malik
Gurdarshan Singh
Gurdarshan
Singh
Parduman R. Sharma
Parduman R.
Sharma
Amal Kaddoumi
Amal
Kaddoumi
Sandip B. Bharate
Sandip B.
Bharate
Ram A. Vishwakarma
Ram A.
Vishwakarma
Ajay Kumar
Ajay
Kumar
Alborixin clears amyloid-β by inducing autophagy through PTEN-mediated inhibition of the AKT pathway
Taylor & Francis Group
2019
Alborixin
alzheimer disease
amyloid beta
autophagy
microglial cells
primary neuronal cells
2019-08-17 07:55:05
Journal contribution
https://tandf.figshare.com/articles/journal_contribution/Alborixin_clears_amyloid-_by_inducing_autophagy_through_PTEN-mediated_inhibition_of_the_AKT_pathway/7872008
<p>Imbalance in production and clearance of amyloid beta (Aβ) is the primary reason for its deposition in Alzheimer disease. Macroautophagy/autophagy is one of the important mechanisms for clearance of both intracellular and extracellular Aβ. Here, through screening, we identified alborixin, an ionophore, as a potent inducer of autophagy. We found that autophagy induced by alborixin substantially cleared Aβ in microglia and primary neuronal cells. Induction of autophagy was accompanied by up regulation of autophagy proteins BECN1/Beclin 1, ATG5, ATG7 and increased lysosomal activities. Autophagy induced by alborixin was associated with inhibition of the phosphoinositide 3-kinase (PI3K)-AKT pathway. A knock down of <i>PTEN</i> and consistent, constitutive activation of <i>AKT</i> inhibited alborixin-induced autophagy and consequent clearance of Aβ. Furthermore, clearance of Aβ by alborixin led to significant reduction of Aβ-mediated cytotoxicity in primary neurons and differentiated N2a cells. Thus, our findings put forward alborixin as a potential anti-Alzheimer therapeutic lead.</p> <p>Aβ: amyloid beta; ALB: alborixin; ATG: autophagy-related; BECN1: beclin 1; DAPI: 4, 6-diamidino-2-phenylindole; DCFH-DA: 2,7-dichlorodihydrofluorescein diacetate; fAβ: fibrillary form of amyloid beta; GFAP: glial fibrillary acidic protein; MAP1LC3B/LC3B: microtubule-associated protein 1 light chain 3 beta; MAP2: microtubule-associated protein 2; MTOR: mechanistic target of rapamycin kinase; PTEN: phosphatase and tensin homolog; ROS: reactive oxygen species; SQSTM1: sequestosome 1; TMRE: tetramethylrhodamine, ethyl ester</p>