10.6084/m9.figshare.7887863.v1 Giuseppina Sanna Giuseppina Sanna Silvia Madeddu Silvia Madeddu Alessandra Serra Alessandra Serra David Collu David Collu Thomas Efferth Thomas Efferth F. Lukmanul Hakkim F. Lukmanul Hakkim Luay Rashan Luay Rashan Anti-poliovirus activity of <i>Nerium oleander</i> aqueous extract Taylor & Francis Group 2019 Apocynaceae Nerium oleander hot extract cold extract poliovirus antiviral activity 2019-03-25 17:19:38 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Anti-poliovirus_activity_of_i_Nerium_oleander_i_aqueous_extract/7887863 <p><i>Nerium oleander</i> (NO), a member of the Apocynaceae family, is an ornamental plant. In this study, we evaluated the antiviral activity of hot and cold extract of NO against six different viruses such as herpes simplex virus type 1 (HSV-1), polio virus type 1 (Sb-1), vesicular stomatitis virus (VSV), reovirus type-1 (Reo-1), human immunodeficiency virus type-1 (HIV-1), and yellow fever virus (YFV). Interestingly the results of plaque reduction assay demonstrated that both, hot extract and cold extract (breastin) of NO inhibited Sb-1 viral infection.</p> <p>In order to identify the mechanism by which NO exerts its antiviral activity, the virucidal effect, the time of addition and the adsorption assay were carried out. Results demonstrated that NO exerts its effect after infection period, particularly during the first two hours post infection.</p>