10.6084/m9.figshare.7908134.v1
Manoj Kumar
Manoj
Kumar
Abhishek Kumar Dwivedy
Abhishek Kumar
Dwivedy
Parismita Sarma
Parismita
Sarma
M. S. Dkhar
M. S.
Dkhar
H. Kayang
H.
Kayang
Richa Raghuwanshi
Richa
Raghuwanshi
Nawal Kishore Dubey
Nawal Kishore
Dubey
Chemically characterised <i>Artemisia nilagirica</i> (Clarke) Pamp. essential oil as a safe plant-based preservative and shelf-life enhancer of millets against fungal and aflatoxin contamination and lipid peroxidation
Taylor & Francis Group
2019
Antifungal
antiaflatoxigenic
antioxidant
essential oil
Artemisia nilagirica
2019-03-28 13:34:43
Journal contribution
https://tandf.figshare.com/articles/journal_contribution/Chemically_characterised_i_Artemisia_nilagirica_i_Clarke_Pamp_essential_oil_as_a_safe_plant-based_preservative_and_shelf-life_enhancer_of_millets_against_fungal_and_aflatoxin_contamination_and_lipid_peroxidation/7908134
<p>The study recommends the <i>Artemisia nilagirica</i> (Clarke) Pamp. essential oil (ANEO) as plant-based shelf-life enhancer of millets against fungal, aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) contamination and lipid peroxidation with favourable safety profile. Chemical characterisation of ANEO through GC-MS, recorded 1,5-heptadiene-4-one,3,3,6-trimethyl (32.72%)as the main compound, followed by Artemisia alcohol (13.40%), alpha lonone (4.55%), benzene, methyl (1-methylethyl) (2.97%) and 1-cyclohexene-1-carboxaldehyde,4-(1-methylethyenyl) (2.23%). The mycoflora analysis of millet samples showed <i>Aspergillus flavus</i> strain[LHP(R)-5] as the most AFB<sub>1</sub> secreting strain. The ANEO inhibited growth and AFB<sub>1</sub> production by the toxigenic strain at 1.4 and 1.0 µL mL<sup>−1</sup>, respectively, and also possess broad fungitoxic spectrum. The decrement in membrane ergosterol content, enhanced leakage of cellular Ca<sup>2+</sup>, K<sup>+</sup> and Mg<sup>2+</sup> ions along with SEM and TEM study of ANEO-treated cell denotes plasma membrane as antifungal site of action. The ANEO also showed strong antioxidant activity as determined by DPPH<sup>•</sup> and ABTS<sup>•+</sup> assays having IC<sub>50</sub> value 2.51 and 1.07 µL mL<sup>−1</sup>, respectively. More than 70.78% protection of Ragi samples from fungal contamination was observed during <i>in situ</i> trial. The ANEO showed favourable safety profile with high LD<sub>50</sub> value (7528.10 µL kg<sup>−1</sup>) for male mice and also exhibited non-phytotoxicity for Ragi seeds germination.</p>