Kumar, Manoj Dwivedy, Abhishek Kumar Sarma, Parismita Dkhar, M. S. Kayang, H. Raghuwanshi, Richa Dubey, Nawal Kishore Chemically characterised <i>Artemisia nilagirica</i> (Clarke) Pamp. essential oil as a safe plant-based preservative and shelf-life enhancer of millets against fungal and aflatoxin contamination and lipid peroxidation <p>The study recommends the <i>Artemisia nilagirica</i> (Clarke) Pamp. essential oil (ANEO) as plant-based shelf-life enhancer of millets against fungal, aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) contamination and lipid peroxidation with favourable safety profile. Chemical characterisation of ANEO through GC-MS, recorded 1,5-heptadiene-4-one,3,3,6-trimethyl (32.72%)as the main compound, followed by Artemisia alcohol (13.40%), alpha lonone (4.55%), benzene, methyl (1-methylethyl) (2.97%) and 1-cyclohexene-1-carboxaldehyde,4-(1-methylethyenyl) (2.23%). The mycoflora analysis of millet samples showed <i>Aspergillus flavus</i> strain[LHP(R)-5] as the most AFB<sub>1</sub> secreting strain. The ANEO inhibited growth and AFB<sub>1</sub> production by the toxigenic strain at 1.4 and 1.0 µL mL<sup>−1</sup>, respectively, and also possess broad fungitoxic spectrum. The decrement in membrane ergosterol content, enhanced leakage of cellular Ca<sup>2+</sup>, K<sup>+</sup> and Mg<sup>2+</sup> ions along with SEM and TEM study of ANEO-treated cell denotes plasma membrane as antifungal site of action. The ANEO also showed strong antioxidant activity as determined by DPPH<sup>•</sup> and ABTS<sup>•+</sup> assays having IC<sub>50</sub> value 2.51 and 1.07 µL mL<sup>−1</sup>, respectively. More than 70.78% protection of Ragi samples from fungal contamination was observed during <i>in situ</i> trial. The ANEO showed favourable safety profile with high LD<sub>50</sub> value (7528.10 µL kg<sup>−1</sup>) for male mice and also exhibited non-phytotoxicity for Ragi seeds germination.</p> Antifungal;antiaflatoxigenic;antioxidant;essential oil;Artemisia nilagirica 2019-03-28
    https://tandf.figshare.com/articles/journal_contribution/Chemically_characterised_i_Artemisia_nilagirica_i_Clarke_Pamp_essential_oil_as_a_safe_plant-based_preservative_and_shelf-life_enhancer_of_millets_against_fungal_and_aflatoxin_contamination_and_lipid_peroxidation/7908134
10.6084/m9.figshare.7908134.v1