Jiang, Haiqin Sun, Jiya Chen, Yanqing Chen, Zhiming Wang, Le Gao, Wei Shi, Ying Zhang, Wenyue Mei, Youming Chokkakula, Santosh Vissa, Varalakshmi Jiang, Taijiao Wu, Aiping Wang, Hongsheng Landscape of the genome and host cell response of <i>Mycobacterium shigaense</i> reveals pathogenic features <p>A systems approach was used to explore the genome and transcriptome of <i>Mycobacterium shigaense</i>, a new opportunistic pathogen isolated from a patient with a skin infection, and the host response transcriptome was assessed using a macrophage infection model. The <i>M</i>. <i>shigaense</i> genome comprises 5,207,883 bp, with 67.2% G+C content and 5098 predicted coding genes. Evolutionarily, the bacterium belongs to a cluster in the phylogenetic tree along with three target opportunistic pathogenic strains, namely, <i>M</i>. <i>avium</i>, <i>M</i>. <i>triplex</i> and <i>M</i>. <i>simiae</i>. Potential virulence genes are indeed expressed by <i>M</i>. <i>shigaense</i> under culture conditions. Phenotypically, <i>M</i>. <i>shigaense</i> had similar infection and replication capacities in a macrophage model as the opportunistic species compared to <i>M</i>. <i>tuberculosis</i>. <i>M</i>. <i>shigaense</i> activated NF-κB, TNF, cytokines and chemokines in the host innate immune-related signaling pathways and elicited an early response shared with pathogenic bacilli except <i>M</i>. <i>tuberculosis</i>. <i>M</i>. <i>shigaense</i> upregulated specific host response genes such as <i>TLR7</i>, <i>CCL4</i> and <i>CXCL5</i>. We performed an integrated and comparative analysis of <i>M</i>. <i>shigaense</i>. Multigroup comparison indicated certain differences with typical pathogenic bacilli in terms of gene features and the macrophage response.</p> typical pathogenic bacilli;related signaling pathways;potential virulence genes;phylogenetic tree along;pathogenic bacilli except;opportunistic species compared;host innate immune;host cell response;early response shared;reveals pathogenic features;host response transcriptome;genome comprises 5;mycobacterium shigaense </;macrophage infection model;macrophage model;shigaense </;gene features;skin infection;similar infection;tuberculosis </;triplex </;tlr7 </;systems approach;simiae </;replication capacities;indeed expressed;cxcl5 </;culture conditions;comparative analysis;ccl4 </;c content;bacterium belongs;avium </;assessed using;activated nf;883 bp 2019-04-02
    https://tandf.figshare.com/articles/dataset/Landscape_of_the_genome_and_host_cell_response_of_i_Mycobacterium_shigaense_i_reveals_pathogenic_features/7936865
10.6084/m9.figshare.7936865.v1