10.6084/m9.figshare.7962548.v1
Julia Lanner
Julia
Lanner
Manuel Curto
Manuel
Curto
Bärbel Pachinger
Bärbel
Pachinger
Ulrich Neumüller
Ulrich
Neumüller
Harald Meimberg
Harald
Meimberg
Illumina midi-barcodes: quality proof and applications
Taylor & Francis Group
2019
DNA barcoding
high-throughput sequencing
Sanger sequencing
CO1 gene
wild bees
2019-04-06 06:25:23
Journal contribution
https://tandf.figshare.com/articles/journal_contribution/Illumina_midi-barcodes_quality_proof_and_applications/7962548
<p>DNA barcoding constitutes a supplemental genetically based characterization tool for the identification of species. Traditionally, the barcodes are generated with a length of 650 bp using standardized Sanger sequencing, but with the introduction of high-throughput sequencing (HTS) methods new opportunities for sequencing are available. To use HTS for barcode collection and identification, the amplification of shorter fragments is preferred. Reference DNA midi-barcodes of wild bees were produced using the Illumina MiSeq as well as the Sanger method. Although DNA midi-barcodes derived from Illumina were comparatively shorter (418 bp), their sequences were coherent to the morphological assignment of species. The Illumina barcodes proved to be effective and dealt better with some general limitations of DNA barcoding.</p>