10.6084/m9.figshare.7992224.v1
Rafael Splendore de Borba
Rafael Splendore
de Borba
Sandra Mariotto
Sandra
Mariotto
Liano Centofante
Liano
Centofante
Claudio Henrique Zawadzki
Claudio Henrique
Zawadzki
Patricia Pasquali Parise-Maltempi
Patricia Pasquali
Parise-Maltempi
Molecular discrimination of <i>Ancistrus</i> lineages (Siluriformes: Loricariidae) using barcode DNA tool
Taylor & Francis Group
2019
Ancistrini
Catfish
distance tree
mitochondrial DNA
taxonomy
2019-04-14 09:42:54
Dataset
https://tandf.figshare.com/articles/dataset/Molecular_discrimination_of_i_Ancistrus_i_lineages_Siluriformes_Loricariidae_using_barcode_DNA_tool/7992224
<p>Although several species of <i>Ancistrus</i> have been described from the Amazon and Paraguay river basins in the states of Amazonas and Mato Grosso, Brazil, the taxonomic status of most specimens from these regions remains doubtful. In the present work, cytogenetic and molecular data were used to discriminate and isolate unexpected <i>Ancistrus</i> lineages from the Amazon and Paraguay basins. For that, it was used DNA barcoding based on mitochondrial Cytochrome Oxidase Subunit I (COI) gene and cytogenic data to perform such molecular discrimination. The analyzed sequences had 669 bp, of which 171 bp were conserved and 491 bp were variable. The Neighbor-joining and Bayesian analysis revealed 21 distinct groups in topology. The genetic distances within each group was 0.4%, 21 times smaller than the mean distance observed among groups, which was 8.4%. These values showed seven distinct lineages of <i>Ancistrus</i> from the studied points of the Amazon basin and eight lineages from the Paraguay basin points. Our results illustrate the efficiency of this technique for the discrimination of the <i>Ancistrus</i> lineages once it indicates the occurrence of cryptic species in these regions, which cannot yet be identified either with just chromosomal or morphological analyzes.</p>