10.6084/m9.figshare.8024933.v1
Parvaneh Eskandari
Parvaneh
Eskandari
Bahare Bigdeli
Bahare
Bigdeli
Mohammad Porgham Daryasari
Mohammad Porgham
Daryasari
Hadi Baharifar
Hadi
Baharifar
Behroz Bazri
Behroz
Bazri
Mostafa Shourian
Mostafa
Shourian
Amir Amani
Amir
Amani
Armin Sadighi
Armin
Sadighi
Bahram Goliaei
Bahram
Goliaei
Mehdi Khoobi
Mehdi
Khoobi
Ali A. Saboury
Ali A.
Saboury
Gold-capped mesoporous silica nanoparticles as an excellent enzyme-responsive nanocarrier for controlled doxorubicin delivery
Taylor & Francis Group
2019
MMP-cleavable peptides
mesoporous silica nanoparticle
Enzyme-responsive nanocarriers
Stimuli-responsive nanocarriers
2019-04-23 07:22:51
Journal contribution
https://tandf.figshare.com/articles/journal_contribution/Gold-capped_mesoporous_silica_nanoparticles_as_an_excellent_enzyme-responsive_nanocarrier_for_controlled_doxorubicin_delivery/8024933
<p>Mesoporous silica nanoparticles (MSNs) have ideal characteristics as next generation of controlled drug delivery systems. In this study, a MSN-based nanocarrier was fabricated and gold nanoparticle (GNP)-biotin conjugates were successfully grafted onto the pore outlets of the prepared MSN. This bioconjugate served as a capping agent with a peptide-cleavable linker sensitive to matrix metalloproteinases (MMPs), which are overexpressed extracellular proteolytic enzymes in cancerous tissue. The prepared nanocarriers were fully characterised by scanning electron microscopy (SEM), transmission electron microscopy (TEM), nitrogen adsorption/desorption, Fourier transform infra-red spectroscopy (FTIR), dynamic light scattering (DLS) and thermo gravimetric analysis (TGA). <i>In vitro</i> release studies showed efficient capping of MSNs with gold gate and controlled release of Doxorubicin (DOX) in the presence of matrix metalloproteinase-2 (MMP-2) and acidic pH values. High DOX-loading capacity (21%) and encapsulation efficiency (95.5%) were achieved using fluorescence technique. DOX-loaded nanocarriers showed high cytocompatibility and could efficiently induce cell death and apoptosis in the MMP-2 overexpressed cell lines. Moreover, Haemolysis, platelet activation and inflammatory responses assessment approved excellent hemocompatibility and minimal side effects by encapsulation of DOX in MSNs carrier.</p>