Evaluation of droplet-based microfluidic platforms as a convenient tool for lipases and esterases assays Pawel Jankowski Adam Samborski Ryszard Ostaszewski Piotr Garstecki 10.6084/m9.figshare.8035634.v2 https://tandf.figshare.com/articles/journal_contribution/Evaluation_of_droplet-based_microfluidic_platforms_as_a_convenient_tool_for_lipases_and_esterases_assays/8035634 <p>The accurate estimation of kinetic parameters is of fundamental importance for biochemical studies for research and industry. In this paper, we demonstrate the application of a modular microfluidic system for execution of enzyme assays that allow determining the kinetic parameters of the enzymatic reactions such as <i>V<sub>max</sub></i> – the maximum rate of reaction and <i>K<sub>M</sub></i> – the Michaelis constant. For experiments, the fluorogenic carbonate as a probe for a rapid determination of the kinetic parameters of hydrolases, such as lipases and esterases, was used. The microfluidic system together with the method described yields the kinetic constants calculated from the concentration of enzymatic product changes via a Michaelis–Menten model using the Lambert function <i>W(x)</i>. This modular microfluidic system was validated on three selected enzymes (hydrolases).</p> 2019-07-23 16:20:48 Enzyme assay lipases esterase microfluidics kinetic enzymatic reactions Lambert function